The Centre for Infectious Disease Research in Zambia (CIDRZ), Central Laboratory is transitioning gene sequencing from the Sanger method to the Illumina MiSeq and the Oxford Nanopore MinION technology – Next Generation Sequencing (NGS), with expanded capabilities for infectious disease surveillance.
NGS is a high throughput technology used to determine the order of nucleotides in entire genomes or targeted regions of DNA or RNA. It is a faster and scalable sequencing technology for various organisms, including faster whole genome sequencing of the SARS COV-2 and identification of mutations and new variants.
The technology is more sensitive in detection of minority variants which cannot be detected by traditional methods of sequencing. For instance, in HIV genotyping the NGS is more sensitive than traditional Sanger method as it can detect drug resistance minority variants which have been shown to contribute to ART failure.
Further, when conducting HIV drug resistance national surveillance, the NGS technology will be faster and lower cost due to the technology’s capability to batch multiple samples. In addition, the introduction of dolutergravir on the first line regimen means CIDRZ Central Laboratory can monitor the HIV drug resistance to integrase. This involves sequencing of the three regions of the HIV genome protease, reverse transcriptase and integrase.
NGS is faster and effective, it reduces the turnaround time to detect drug resistance Tuberculosis within 96 hours compared to the traditional method of culture which takes longer than three weeks.
With the NGS technology CIDRZ is able to run tests for HIV drug resistance, Tuberculosis drug resistance and identification and characterization of antimicrobial resistance.
As part of our training protocols and optimisation of methods, we successfully sequenced Campylobacter species and will soon complete sequencing of Echerichia coli, and Klebsiella pneumoniae.
The benefits of the NGS technology to our clients include: